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B-Cell Leukemia/Lymphoma 2 Human Recombinant (-BH4)
| 目录号 | AP1863 |
|---|---|
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The protein contains 10mM Tris-HCl pH-8, 1mM EDTA and 250mM NaCl. |
| Uniprot ACC# | P10415 |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Solubility | Suspend Bcl-2 in 100µl of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended. |
| Stability | Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Source | Escherichia Coli. |
| Synonyms | Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2. |
| Transportation method | Shipped at Room temp |
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