详情
Granulocyte Macrophage-Colony Stimulating Factor Human Recombinant
目录号 | AP3352 |
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Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4??0.1. |
Protein biological activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11,100,000 IU/mg. |
Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.N-terminal methionine has been completely removed enzymatically. |
Uniprot ACC# | P04141 |
Purity | Greater than 98.0% as determined by:1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE. |
Solubility | It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M??-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability | Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
Source | Escherichia Coli. |
Synonyms | CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897. |
Transportation method | Shipped at Room temp |
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