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Matrix Metalloproteinase-1 Human Recombinant, HEK
| 目录号 | AP4225 |
|---|---|
| Physical Appearance | Sterile Filtered colorless solution. |
| Formulation | The MMP-1 is supplied as a 0.2µm filtered solution in MES, NaCl, Glycerol and Brij35. |
| Protein biological activity | The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is > 400 pmoles/min/µg.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP1 to 50µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 2 hours. |
| Uniprot ACC# | P03956 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles. |
| Source | HEK293 cells. |
| Synonyms | Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, MMP1, CLG, CLGN. |
| Transportation method | Shipped with Ice Packs |
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