MMP 1 Human, HEK

Catalog No.: AP4225

产品概述

Matrix Metalloproteinase-1 Human Recombinant, HEK

Product Details

MMP-1 Human Recombinant produced in HEK293 cells is a proform of the Human MMP1 (Met1-Asn469) and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. MMP-1 is purified by proprietary chromatographic techniques.

库存: 现货

规格 价格 数量
MMP 1 Human, HEK 2µg
¥ 770.00
MMP 1 Human, HEK 10µg
¥ 2,030.00
MMP 1 Human, HEK 1mg
¥ 72,100.00

详情

Matrix Metalloproteinase-1 Human Recombinant, HEK
目录号 AP4225
Physical Appearance Sterile Filtered colorless solution.
Formulation The MMP-1 is supplied as a 0.2µm filtered solution in MES, NaCl, Glycerol and Brij35.
Protein biological activity The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is > 400 pmoles/min/µg.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP1 to 50µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 2 hours.
Uniprot ACC# P03956
Purity Greater than 95% as determined by SDS-PAGE.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.
Source HEK293 cells.
Synonyms Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, MMP1, CLG, CLGN.
Transportation method Shipped with Ice Packs

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