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Nephrosis 2 Idiopathic Steroid-Resistant Human Recombinant
| 目录号 | AP1283 |
|---|---|
| Physical Appearance | Filtered White lyophilized (freeze-dried) powder. |
| Formulation | NPHS2 was filtered (0.4µm) and lyophilized in 30mM acetate buffer and 1mM dithiothreitol, pH 4. |
| Amino acid sequence | MKHHHHHHASVKVVQEYERV IIFRLGHLLP GRAKGPGLFF FLPCLDTYHK VDLRLQTLEI PFHEIVTKDM FIMEIDAICY YRMENASLLL SSLAHVSKAV QFLVQTTMKR LLAHRSLTEI LLERKSIAQD AKVALDSVTC IWGIKVERIE IKDVRLPAGL QHSLAVEAEA QRQAKVRMIA AEAEKAASES LRMAAEILSG TPAAVQLRYL HTLQSLSTEK PSTVVLPLPF DLLNCLSSPS NRTQGSLPFP SPSKPVEPLN PKKKDSPML. |
| Uniprot ACC# | Q9NP85 |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Solubility | It is recommended to add 0.1M acetate buffer pH 4.0 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. NPHS2 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Source | Escherichia Coli. |
| Synonyms | Nephrosis 2, Idiopathic, Steroid-Resistant (Podocin), PDCN, SRN1, Podocin, NPHS2. |
| Transportation method | Shipped at Room temp |
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