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Parvalbumin Rat Recombinant
| 目录号 | AP1854 |
|---|---|
| Applications | The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For fluid-phase adsorption in immunohistochemistry we suggest the following procedure:A- Dilute 1 µl of the monoclonal antibody 235 against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).B- Add 1µg of the Parvalbumin to 1ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The protein was lyophilized from a concentrated solution (1mg/ml) without any additives. |
| Uniprot ACC# | P02625 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Solubility | It is recommended to reconstitute the lyophilized Parvalbumin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Parvalbumin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Parvalbumin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Source | Escherichia Coli. |
| Synonyms | Pvalb, Pva, PALB1, Parvalbumin. |
| Transportation method | Shipped at Room temp |
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