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5(6)-Carboxyfluorescein contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid; cell-impermeant 5,6-FAM can also be used as a nonfixable polar tracer to investigate fusion, lysis and gap-junctional communication and to detect changes in cell or liposome volume.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 26.57 mL | 132.87 mL | 265.73 mL |
| 0.5 mM | 5.31 mL | 26.57 mL | 53.15 mL |
| 1 mM | 2.66 mL | 13.29 mL | 26.57 mL |
| 5 mM | 0.53 mL | 2.66 mL | 5.31 mL |
*The above data is based on the productmolecular weight 376.32. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 目录号 | A21120 |
|---|---|
| M. Wt | 376.32 |
| Formula | C21H12O7 |
| Purity | >99% |
| Storage | Store lyophilized at -20ºC, keep desiccated. |
| CAS No. | 72088-94-9 |
| Synonyms | 5(6)-FAM; 5-(and-6)-Carboxyfluorescein mixed isomers |
| SMILES | OC1=CC=C(C2(O3)C(C=C(C(O)=O)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(C(O)=O)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6 |
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