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Jun Proto-Oncogene Human Recombinant
| 目录号 | AP4157 |
|---|---|
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | C-JUN is supplied as lyophilized powder containing no additives. |
| Protein biological activity | C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application. |
| Uniprot ACC# | P05412 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Solubility | It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to a concentration of 0.2-1.0 mg/ml. |
| Stability | Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Source | Escherichia Coli. |
| Synonyms | Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, c-Jun. |
| Transportation method | Shipped at Room temp |
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