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Mitogen Activated Kinase Kinase 1 Human Recombinant
| 目录号 | AP4632 |
|---|---|
| Physical Appearance | Sterile Filtered clear solution. |
| Formulation | MEK1 is supplied at a concentration of in 40mM Tris, pH-8, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35. |
| Protein biological activity | ~125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30°C in a reaction containing 100µM ATP. Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. |
| Uniprot ACC# | Q02750 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Stability | Store at 4°C if entire vial will be used within 1-2 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Source | Sf9 Insect Cells. |
| Synonyms | MEK1, MKK1, MAPKK1, MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, EC 2.7.12.2, ERK activator kinase 1, MAPK/ERK kinase 1, PRKMK1. |
| Transportation method | Shipped with Ice Packs |
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