Pfu DNA Polymerase

Catalog No.: AP3983

产品概述

Pfu-DNA Polymerase Recombinant

Product Details

Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5? to 3? direction in the existence of magnesium. Pfu DNA Polymerase possesses 3? to 5? exonuclease (proofreading) activity. Base misinsertions that take place during polymerization are swiftly removed by the proofreading activity of the polymerase. Therefore, Pfu DNA Polymerase is suggested for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.

库存: 现货

规格 价格 数量
Pfu DNA Polymerase 100U
¥ 2,030.00
Pfu DNA Polymerase 500U
¥ 3,570.00
Pfu DNA Polymerase 2500U
¥ 14,210.00

详情

Pfu-DNA Polymerase Recombinant
目录号 AP3983
Applications 1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3?? overhangs. 5. Optimal for blunt-end PCR cloning. 6. Optimum temperature near 75°C. 7. 95% active after 1-hour incubation at 98°C.
Physical Appearance Sterile liquid formulation.
Formulation 50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol.
Uniprot ACC# P61875
Stability Pfu DNA Polymerase although stable at 10°C for 5 days, should be stored below -18°C.Please prevent freeze-thaw cycles.
Source Escherichia Coli.
Synonyms DNA polymerase, EC 2.7.7.7, Pfu polymerase, Pfu-DNA Polymerase.
Transportation method Shipped with Ice Packs

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