Urease

Catalog No.: AP4308

产品概述

Urease Recombinant

Product Details

Urease (EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: (NH2)2CO + H2O = CO2 + 2NH3. In 1926 James Sumner showed that urease is a protein. Urease is found in bacteria, yeast and several higher plants. Characteristics: Active site metal: nickel(II); Molecular weight: 480 kDa or 545 kDa for Jack Bean Urease (calculated mass from the amino acid sequence); Optimum pH: 7.4; Optimum Temperature: 60 degrees Celsius; Enzymatic specificity: urea; Inhibitors: heavy metals. The multi-subunit enzyme usually has a 3:3 (alpha:beta) stoichiometry with a 2-fold symmetric structure (note that the image above gives the structure of the asymmetric unit, one third of the true biological assembly). An exceptional urease is found in Helicobacter pylori, which combines four of the regular six subunit enzymes in an overall tetrahedral assembly of 24 subunits (a12b12). This supra-molecular assembly is thought to confer additional stability for the enzyme in this organism, which functions to produce ammonia in order to neutralise gastric acid. The presence of urease is used in the diagnosis of Helicobacter species.

库存: 现货

规格 价格 数量
Urease 1mg
¥ 770.00
Urease 5mg
¥ 2,030.00
Urease 50mg
¥ 15,120.00

详情

Urease Recombinant
目录号 AP4308
Physical Appearance Sterile Lyophilized Powder.
Formulation Each mg of protein contains 420µg Potassium Phosphate and 30µg EDTA Na2.
Protein biological activity The activity was found to be 141U/mg powder.
Uniprot ACC# 0
Purity Greater than 95.0% as determined by SDS-PAGE.
Solubility It is recommended to reconstitute the lyophilized Urease in sterile 18M??-cm H2O.
Stability Urease although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Source Escherichia Coli.
Transportation method Shipped at Room temp

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